HMGB1 Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) was expressed with the fused Fc region of human IgG1 at the N-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Glu215

标签类型：N-hFc

分子量：The recombinant human HMGB1/Fc is a disulfide-linked homodimeric protein.The reduced monomer consists of 452 amino acids and has a predictedmolecular mass of 51.5 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1/Fc monomer is approximately 55-60 kDa inSDS-PAGE under reducing conditions.

活性：Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse AGER at 2 μg/ml (100 μl/well) can bind human HMGB1. The ECof human HMGB1 is 0.23 μg/ml.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：high mobility group box 1

通用名：HMGB1

蛋白序参考：NP_002119.1

总结：High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2, and HMGB3. HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. It is the prototypic damage-associated molecular pattern (DAMP) molecule and has been implicated in several inflammatory disorders. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis are present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription, and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses. Extracellular HMGB1 represents an optimal " necrotic marker" selected by the innate immune system to recognize tissue damage and initiate reparative responses. However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis. As shown in studies on patients as well as animal models, HMGB1 can play an important role in the pathogenesis of the rheumatic disease, including rheumatoid arthritis, systemic lupus erythematosus, and polymyositis among others. Besides, enhanced postmyocardial infarction remodeling in type 1 diabetes mellitus was partially mediated by HMGB1 activation.

形式：Lyophilized

纯度：> 97 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：HMG-1 Protein, Human; HMG1 Protein, Human; HMG3 Protein, Human; SBP-1 Protein, Human