Stable for 1 year at -989°C or below from date of shipment.
保质期:
1-2年
供应商:
安诺伦(北京)生物科技有限公司
规格:
50 ug
蛋白长度:A DNA sequence encoding the human VEGF 121 isoform b (P15692-9) (Met1-Asp147,with last 6 amino acids switch from CDKPRRtoSLTRKD ) was expressed.
性质:
表达系统:HEK293 Cells
种属:Human
氨基酸序:Met1-Asp147
标签类型:No tag
分子量:The recombinant human VEGF consists of 121 amino acids and has a predicted molecular mass of 14 kDa. The apparent molecular mass of the human VEGF121b is approximately 20 and 16 kDa in SDS-PAGE under reducing conditions due to glycosylation.
活性:1. Measured by its binding ability in a functional ELISA. Immobilized human VEGF121b(Cat:PH50969M0) at 10μg/mL (100μL/well) can bind human VEGFR2-Fc(Cat:PH50475M4), the ECof human VEGFR2-Fc is 0.06-0.1 μg/mL.2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The EDfor this effect is typically 1-5ng/mL.
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称:vascular endothelial growth factor A
通用名:VEGFA
蛋白序参考:
总结:Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.
形式:Lyophilized
纯度:> 95 % as determined by SDS-PAGE.
储存条件:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.Please refer to the specific buffer information in the hard copy of CoA.