Stable for 1 year at -974°C or below from date of shipment.
保质期:
1-2年
供应商:
安诺伦(北京)生物科技有限公司
规格:
100 ug
蛋白长度:A DNA sequence encoding the mature form of human PDGF-C (NP_057289.1) (Val 235-Gly 345) was expressed with the fused Fc region of human IgG1 at the N-terminus.
性质:
表达系统:HEK293 Cells
种属:Human
氨基酸序:Val235-Gly345
标签类型:N-hFc
分子量:The recombinant human PDGFC/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 348 amino acids and predicts a molecular mass of 39 kDa. As a result of glycosylation, rh PDGFC/Fc monomer is approximately 45 kDa in SDS-PAGE under reducing conditions.
活性:Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.The EDfor this effect is typically 0.5-3 μg/mL.
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称:platelet derived growth factor C
通用名:PDGFC
蛋白序参考:NP_057289.1
总结:PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique domain organization and expression pattern. Platelet-derived growth factor receptors (PDGFRs) are catalytic receptors that have intracellular tyrosine kinase activity. They have roles in the regulation of many biological processes including embryonic development, angiogenesis, cell proliferation and differentiation, and contribute to the pathophysiology of some diseases, including cancer. There are two isoforms of the PDGFR receptor; PDGFRalpha and PDGFRbeta, which can form homo- or heterodimers. The endogenous PDGFR ligands are PDGF-A, -B, -C and -D, which induce receptor dimerization and transphosphorylation at specific tyrosine residues upon binding. This activates the intracellular kinase activity, initiating intracellular signaling through the MAPK, PI 3-K and PKCgamma pathways. PDGF-C acts as a specific ligand for alpha platelet-derived growth factor receptor homodimer, and alpha and beta heterodimer. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. PDGF-C appears to be involved in the three stages of wound healing: inflammation, proliferation and remodeling. PDGF-C is involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs.
形式:Lyophilized
纯度:> 97 % as determined by SDS-PAGE.
储存条件:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5Please contact us for any concerns or special requirements.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.Please refer to the specific buffer information in the hard copy of CoA.