Carbonic Anhydrase X Protein,

蛋白长度：The mature form of human CARPX (NP_001076002.1) (Met 1-Asn 300) with five amino acids (DDDDK) at the C-terminus was expressed and purified.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Asn300

标签类型：No tag

分子量：The recombinant human CARPX consists of 285 amino acids and has a predicted molecular mass of 27.2 kDa. Due to glycosylation, the rhCARPX migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions.

活性：Measured by its esterase activity. The specific activity is >100 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：carbonic anhydrase X

通用名：CA10

蛋白序参考：NP_001076002.1

总结：Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA1, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：CA-RPX Protein, Human; CA10 Protein, Human; CARPX Protein, Human; HUCEP-15 Protein, Human