EphB2 Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the human EPHB2 (NP_059145.2) (Met 1-Leu 543) was expressed with six amino acids (ENLYFQ ) at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Leu543

标签类型：No tag

分子量：The recombinant human EPHB2 consists of 532 amino acids and predicts a molecular mass of 59 KDa. It migrates as an approximately 66 KDa band in SDS-PAGE under reducing conditions.

活性：Immobilized human EPHB2 at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch (Cat:10881-H03H), The ECof human EFNB2-Fch (Cat:10881-H03H) is 18.2-42.7 ng/ml.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：EPH receptor B2

通用名：EphB2

蛋白序参考：NP_059145.2

总结：Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：CAPB Protein, Human; DRT Protein, Human; EK5 Protein, Human; EPHT3 Protein, Human; ERK Protein, Human; Hek5 Protein, Human; PCBC Protein, Human; Tyro5 Protein, Human