PD-L1 Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the human CD274 (NP_054862.1) (Met1-Thr239) was expressed with C-terminal fused Fc region of human IgG1. The purified protein was biotinylated in vitro.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Thr239

标签类型：C-hFc

分子量：The recombinant human CD274 consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, it migrates as an approximately 73.6 kDa protein in SDS-PAGE under reducing conditions.

活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：CD274 molecule

通用名：CD274

蛋白序参考：NP_054862.1

总结：Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：B7-H Protein, Human; B7-H1 Protein, Human; B7H1 Protein, Human; PD-L1 Protein, Human; PDCD1L1 Protein, Human; PDCD1LG1 Protein, Human; PDL1 Protein, Human