CD45 Protein, Human, Recombina

蛋白长度：A DNA sequence encoding the human CD45 (NP_563578.2) (Met1-Lys416) was fused with the Fc region of human IgG1 at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Lys416

标签类型：C-hFc

分子量：The recombinant mature human CD45/Fc chimeric protein consists of 629 amino acids and has a calculated molecular mass of 71.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human CD45/Fc monomer is approximately 122 kDa due to glycosylation.

活性：Measured by its ability to bind biotinylated human Galectin-1 (Cat:PH51598E0-E) in a functional ELISA.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：protein tyrosine phosphatase, receptor type, C

通用名：PTPRC

蛋白序参考：NP_563578.2

总结：The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：B220 Protein, Human; CD45 Protein, Human; CD45R Protein, Human; GP180 Protein, Human; L-CA Protein, Human; LCA Protein, Human; LY5 Protein, Human; T200 Protein, Human