Stable for 1 year at -774°C or below from date of shipment.
保质期:
1-2年
供应商:
安诺伦(北京)生物科技有限公司
规格:
50 ug
蛋白长度:A DNA sequence encoding the human RGMB (NP_001012779.2) (Met1-Asn413) was expressed with a polyhistidine tag at the C-terminus.
性质:
表达系统:HEK293 Cells
种属:Human
氨基酸序:Met1-Asn413
标签类型:C-His
分子量:The recombinant human RGMB consists of 379 amino acids and predicts a molecular mass of 42 kDa.
活性:At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA.When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate.We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
内毒素:< 1.0 EU per μg protein as determined by the LAL method.
基因名称:repulsive guidance molecule family member B
通用名:RGMB
蛋白序参考:NP_001012779.2
总结:RGMB was knocked down in breast cancer cells by way of an anti-RGMB ribozyme transgene. Knockdown of RGMB resulted in enhanced capacities of proliferation, adhesion, and migration in breast cancer cells. Further investigations demonstrated RGMB knockdown resulted in a reduced expression and activity of Caspase-3, accompanied with better survival in RGMB knockdown cells under serum starvation, which might be induced by its repression on MAPK JNK pathway. That RGMB may act as a negative regulator in breast cancer through BMP signaling.
形式:Lyophilized
纯度:> 90 % as determined by SDS-PAGE.
储存条件:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.Please refer to the specific buffer information in the hard copy of CoA.