CD39 Protein, Human, Recombina

蛋白长度：A DNA sequence encoding the human ENTPD1 (NP_001767.3) (Thr38-Val478) was expressed with a polyhistidine tag at the C-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Thr38-Val478

标签类型：C-His

分子量：The recombinant human ENTPD1 consists of 452 amino acids and predicts a molecular mass of 51.8 kDa. As a result of glycosylation, it migrates as an approximately 56.7 kDa band in SDS-PAGE under reducing conditions.

活性：Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The specific activity is >5000 pmol/min/μg.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：ectonucleoside triphosphate diphosphohydrolase 1

通用名：ENTPD1

蛋白序参考：NP_001767.3

总结：CD39, also known as ENTPD1, belongs to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. It is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism. There is a correlation between ATP hydrolysis and triglycerides in patients with chronic heart disease, suggesting a relationship between ATP diphosphohydrolase and thrombogenesis. In the nervous system, CD39 could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.

形式：Liquid

纯度：> 90 % as determined by SDS-PAGE.

储存条件：Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Supplied as sterile 20mM Tris, 300mM NaCl, 10% Glycerol, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

别名：ATPDase Protein, Human; CD39 Protein, Human; NTPDase-1 Protein, Human; SPG64 Protein, Human