Stable for 1 year at -580°C or below from date of shipment.
保质期:
1-2年
供应商:
安诺伦(北京)生物科技有限公司
规格:
50 ug
蛋白长度:A DNA sequence encoding the mature form of human ENTPD2 (Q9Y5L3) (Thr29-Asp460) was expressed, with a polyhistidine tag at the N-terminus.
性质:
表达系统:Baculovirus-Insect Cells
种属:Human
氨基酸序:Thr29-Asp460
标签类型:N-His
分子量:The recombinant human human ENTPD2 consists of 448 amino acids and predicts a molecular mass of 49.3 KDa. It migrates as an approximately 59 KDa band in SDS-PAGE under reducing conditions.
活性:Measured by its ability to hydrolyze the 5’phosphate groups from the substrate adenosine 5’triphosphate(ATP).The specific activity is > 5,000 pmoles/min/μg.
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method.
总结:NTPDase 2, also known as ENTPD2, belongs to the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). Members of E-NTPDase family are nucleotidases able to hydrolyze 5′-nucleoside tri- and/or diphosphates; the main role of these enzymes is the termination of purinergic signaling. NTPDases are ubiquitous and were previously shown in other parasites including the trypanosomatides of genus Leishmania and in T. brucei. NTPase activity would act as a timer and is crucial to T. gondii infection. In L. pneumophila it was demonstrated that an E-NTPDase, similar to CD39, is essential for intracellular bacterial multiplication. NTPDase 2 is an integral membrane protein. In the nervous system, it could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Alternative splicing of NTPDase 2 gene results in multiple transcript variants.
形式:Liquid
纯度:> 85 % as determined by SDS-PAGE.
储存条件:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
别名:CD39L1 Protein, Human; NTPDase-2 Protein, Human; RP11-229P13.11-001 Protein, Human