Creatine kinase B Protein, Hum

蛋白长度：A DNA sequence encoding the human CKB (P12277)(Pro2-Lys381) was fused with a polyhistide tag at the N-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Pro2-Lys381

标签类型：N-His

分子量：The recombinant human CKB consists of 398 amino acids and has a calculated molecular mass of 44.8 kDa. The recombinant protein migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.

活性：Kinase activity untested

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：creatine kinase, brain

通用名：CKB

蛋白序参考：

总结：CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc.

形式：Liquid

纯度：> 90 % as determined by SDS-PAGE.

储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Supplied as sterile 50mM Tris, 100mM NaCl, 10% glycerol, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

别名：B-CK Protein, Human; BCK Protein, Human; CKBB Protein, Human; HEL-211 Protein, Human; HEL-S-29 Protein, Human