ErbB4 Protein, Human, Rhesus,

蛋白长度：A DNA sequence encoding the human / rhesus ERBB4 (NP_005226.1) (Met1-Arg649) with a C-terminal polyhistidine tag was expressed. Human and Rhesus ERBB4 sequences are identical.

性质：

表达系统：HEK293 Cells

种属：Human, Rhesus

氨基酸序：Met1-Arg649

标签类型：C-His

分子量：The recombinant human / rhesus ERBB4 comprises 635 amino acids and has a predicted molecular mass of 71.1 kDa. The apparent molecular mass of the protein is approximately 102 kDa in SDS-PAGE under reducing conditions.

活性：Measured by its binding ability in a functional ELISA.Immobilized human ErbB4-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (Cat:PH51626M3), The ECof biotinylated human NRG1 (Cat:PH51626M3) is 0.4-0.92 μg/ml.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：erb-b2 receptor tyrosine kinase 4

通用名：ERBB4

蛋白序参考：NP_005226.1

总结：ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.

形式：Lyophilized

纯度：> 85 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

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