EpCAM Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the extracellular domain (Met1-Lys265) of human EpCAM (NP_002345.1) was fused with a polyhistidine tag at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Lys265

标签类型：C-His

分子量：The recombinant human EpCAM consists of 253 amino acids after removal of the signal peptide and has a calculated molecular mass of 28.84 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human EpCAM is approximately 36.5 kDa due to glycosylation.

活性：Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-His coated plates (1.25μg/mL, 100μL/well), approximately >40% will adhere specifically after 30 minutes at 37℃.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：epithelial cell adhesion molecule

通用名：EpCAM

蛋白序参考：NP_002345.1

总结：Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type a…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

形式：Lyophilized

纯度：> 96 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：DIAR5 Protein, Human; EGP-2 Protein, Human; EGP314 Protein, Human; EGP40 Protein, Human; ESA Protein, Human; HNPCC8 Protein, Human; KS1/4 Protein, Human; KSA Protein, Human; M4S1 Protein, Human; MIC18 Protein, Human; MK-1 Protein, Human; TACSTD1 Protein, Human; TROP-1 Protein, Human; TROP1 Protein, Human