FLRT2 Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the human FLRT2 (O43155) extracellular domain (Met 1-Ser 539) was expressed, with a C-terminal polyhistidine tag.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Ser539

标签类型：C-His

分子量：The secreted recombinant human FLRT2 comprises 515 amino acids and has a calculated molecular mass of 57.7 kDa. The apparent molecular mass of the recombinant protein is approximately 75 kDa in SDS-PAGE under reducing conditions due to glycosylation.

活性：Measured by the ability of the immobilized protein to support the adhesion of Neuro 2A mouse neuroblastoma cells.When cells are added to coated plates(5μg/mL, 100μL/well), approximately 50%-70% will adhere after 1 hour at 37℃.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：fibronectin leucine rich transmembrane protein 2

通用名：FLRT2

蛋白序参考：

总结：Fibronectin Leucine-Rich Transmembrane (FLRT) proteins are glycosylated membrane proteins expressed at the cell surface which localise in a homophilic manner to cell-cell contacts expressing the focal adhesion marker vinculin. FLRT1, FLRT2, and FLRT3, the three genes encode putative type I transmembrane proteins, each containing 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRT family members may function in cell adhesion and/or receptor signalling. Each member of the FLRT family has a distinct, highly regulated expression pattern, as was seen for the NLRR family. FLRT2 is expressed in a subset of the sclerotome, adjacent to the region that forms the syndetome, suggesting that interaction with FGF signalling may be a general property of FLRT proteins. All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. FLRT proteins have a dual role, promoting FGF signalling and modulating homotypic cell adhesion. FLRT2 played critical roles in craniofacial development, and it was also present in the vomero-nasal organ, mandibular primodia, and the posterior aspects of the unfused and fused secondary palatal shelves.

形式：Lyophilized

纯度：> 98 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：KIAA0405 Protein, Human; UNQ232/PRO265 Protein, Human