GBA3 Protein, Human, Recombina

蛋白长度：A DNA sequence encoding the human GBA3 (NP_066024.1) (Met 1-Leu 469) was fused with a polyhistidine tag at the C-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Met1-Leu469

标签类型：C-His

分子量：The recombinant human GBA3 consists of 480 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions.

活性：Measured by its ability to hydrolyze 4-methylumbelliferyl-β-D glucopyranoside.The specific activity is >1,500 pmoles/min/μg.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：glucosidase, beta, acid 3 (gene/pseudogene)

通用名：GBA3

蛋白序参考：NP_066024.1

总结：Cytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to theglycosyl hydrolase 1 family and Klotho subfamily. GBA3 / CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3 / CBGL1 is present in small intestine (at protein level). GBA3 / CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3 / CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3 / CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3 / CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide.

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：CBG Protein, Human; CBGL1 Protein, Human; GBA3 Protein, Human; GLUC Protein, Human; KLrP Protein, Human; MGC104276 Protein, Human; MGC126878 Protein, Human