CD55/DAF Protein, Human, Reco

蛋白长度：A DNA sequence encoding the human CD55 precursor (NP_000565.1) (Met 1-Ser 353) was expressed, with a C-terminal polyhistidine tag.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Ser353

标签类型：C-His

分子量：The secreted recombinant human CD55 consists of 330 amino acids and has a predicted molecular mass of 36.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD55 is approximately 55-60 kDa due to glycosylation.

活性：Immobilized Human CD55 His (Cat: PH50000M2) at 2 μg/ml (100 μl/well) can bind Human CD97 hFc (Cat: PH50839M4), the ECof Human CD97 hFc is 15-65 ng/mL.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：CD55 molecule, decay accelerating factor for complement (Cromer blood group)

通用名：CD55

蛋白序参考：NP_000565.1

总结：CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：CR Protein, Human; CROM Protein, Human; DAF Protein, Human; TC Protein, Human