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文献和实验A general protocol for staining cell for cytometry analysis
) 4.Cells stained with PI and SAv-FITC (no Annexin V-Biotin) Other Staining Controls: A cell line that can be easily induced to undergo apoptosis should be used to obtain positive control staining. It is important to note that the basal level
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
Brief protocol using Digest-All™ Kit is described below. The Digest-All™ is a flexible and standardized system for tissue digestion and contains trypsin, ficin, and pepsin proteolytic enzymes. The degree of digestion is based on a standardized 10 minute
CellTrace CFSE Cell Proliferation Kit
in phosphate-buffered saline (PBS) or other suitable buffer to the desired working concentration (0.5–25 μM). Resuspend the cells gently in prewarmed (37°C) PBS containing the probe (prepared in previous step). Incubate the cells for 15 min
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