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文献和实验Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
LIVE/DEAD® Violet Viability/Vitality Kit
. Material Amount Storage Stability CellTrace™ calcein violet stain, lyophilized (Component A) *
by the addition of a detergent results in the liberation of the crystals which are solubilized. The color can then be quantified using a simple colorimetric assay. The results can be read on a multiwell scanning spectrophotometer (ELISA reader). The MTT Cell
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