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文献和实验Assay of Phospholipase A Activity
are important signal molecules in the cellular adaptation to stress, developmental processes and several diseases in plants and animals. The assay of PLA activity has been much promoted by the availability of phospholipid substrates with fluorophores
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
to the ligation reaction mixture. This procedure is based on the fact that Taq polymerase has a nontemplate dependent activity that adds a single deoxyadenosine to the 3′ ends of double-stranded DNA (2 ). The Invitrogen pCR™II vector is supplied as a linearized
LIVE/DEAD® Violet Viability/Vitality Kit
of labelings: At the recommended reagent concentrations and volumes, this kit contains sufficient material to perform approximately 200 assays using flow cytometry. Approximate fluorescence excitation/emission maxima: calcein violet 400/452 nm; aqua
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