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- 2025年08月25日
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文献和实验在RT-PCR中避免DNA污染（Avoiding DNA Contamination in R
extraction (TRI Reagent®), a filter-binding based extraction (RNAqueous®), by centrifugation through a CsCl cushion, and by two rounds of oligo d(T) selection using Ambion's Poly(A)Pure™ Kit (see Figure 1a). Regardless of whether reverse transcriptase
Antibody Validation by Western Blotting
by chemiluminescent, fluorescent, or colorimetric methods. This chapter focuses on the chemiluminescent detection of proteins using a manual western blotting system and a vacuum-enhanced detection system (SNAP i.d.™, Millipore)
6. Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210) 实验设备 5 mL Culture Tubes 实验步骤 1. Remove liver aseptically. 2. Flash-freeze liver in foil packets using liquid nitrogen. Store
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