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文献和实验Introduction Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent . Some bacteria are naturally competent (e.g B
Bacterial Transformation (Kushner Method)
The principle of this procedure is very similar to that of the standard calcium chloride method, (see Chapter 34 ) i.e., exponential phase cells are harvested and washed with a solution containing divalent cations. This renders the cells
Bacterial Transformation(细菌转化)
相关专题 Introduction Transformation is a technique to introduce DNA into bacterial Cell s. There are many variations on a common theme, but the key points are listed below. Check details with supplier of competent
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