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文献和实验Detection and Quantitation of Uracil DNA Glycosylase Activity
One of the main determining factors for maintaining the informational integrity of the DNA genomes in all organisms is the efficiency of repair of DNA lesions. DNArepair mechanisms have evolved to counteract the deleterious effects of DNA
Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography
matrix (3 –3 ). Affinity chromatography procedures can achieve purification levels on the order of several thousand-fold in a single separation step. The method presented here utilizes the bacteriophage PBS2 uracil-DNA glycosylase inhibitor (Ugi
radical is highly reactive, producing a variety of purine- and pyrimidine-derived lesions in DNA (2 ,3 ). A major pathway of hydroxyl radical-induced DNA damage involves attack on the C8 position of purines to produce 8-oxoG (7,8-dihydro-8-oxoguanine
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