Dazzle™ 594 抗人 FOXP3 荧光标记PE 抗体

Dazzle™ 594 anti-human FOXP3 Antibody-PE详细如下：

Description ：FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin,JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of Tregulatory cells than most cell surface markers (such as CD4 and CD25). Transducedexpression of FOXP3 in CD4 /CD25 cells has been shown to induce GITR, CD103, andCTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunityallergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice.Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggestingthat this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse,two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical fulllength sequence; the other (FOXP3 δ2) lacking exon 2. The 206D antibody recognizes humanFOXP3 epitope in the region of amino acids 105-235.

Verified Reactivity ：Human

Reported Reactivity ：Baboon, Cynomolgus, Rhesus, Pigtailed Macaque

Antibody Type ：Monoclonal

Host Species ：Mouse

Immunogen ：Full-length FOXP3 protein

Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation ：The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.

Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.

Application ：ICFC - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by intracellular flow cytometry using our TrueNuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested useof this reagent is 5 µl per million cells in 100 µl staining volume. It is recommended that the reagentbe titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)