TotalSeq™-B0848 抗小鼠 TIGIT (Vst

TotalSeq™-B0848 anti-mouse TIGIT (Vstm3) Antibody详细如下：

Description ：T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set andtransmembrane domain-containing protein 3 (Vstm3), is a 26 kD, type I transmembrane proteinand member of the CD28 family. TIGIT is expressed on activated T cells, follicular T helper,memory, and regulatory T cells as well as on NK cells. Its binding partners include CD155(PVR) and CD112 (PVRL2). TIGIT is a negative regulator of NK and T cell activation.Engagement of TIGIT by dendritic cells results in their differentiation into a tolerogenicphenotype, with an increased secretion of IL-10 and a diminished production of IL-12. Micedeficient for TIGIT are more susceptible to autoimmune disease.

Verified Reactivity ：Mouse

Antibody Type ：Monoclonal

Host Species ：Mouse

Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.

Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer underoptimal conditions.

Concentration ：0.5 mg/ml

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application ：PG - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.