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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
AbMole
- CAS号:
57-50-1
- 规格:
250g
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文献和实验Sucrose Fractionation of Castor Bean
Materials Castor beans Gradient former Sucrose solutions in 0.01 M EDTA, pH 7.5 33%, 44%, 50%, 57%, 60% (w/v) Grinding Medium 0.4 M sucrose 0.165 M Tris-HCl buffer, pH 7.5 0.01 M KCl
Lysosome Isolation in Isotonic Sucrose
LEVEL I MATERIALS Rat liver Physiological saline (0.85% w/v NaCl) 0.25 M sucrose in 10 mM Tris-HCL, pH 7.4 Brendler teflon homogenizer Refrigerated preparative centrifuge 0.08 M CaCl in 0.25 M
Sucrose Density Gradient Fractionation
to 606 mg (0.606 g)sucrose in a μltracentrifuge tube (this makes a 70% solution)--use tweezers to handle μltracentrifuge tubes,add "flea" (the tiny stir bar).Put a bunch of tubes in a small beaker and put the beaker on a stir plate in the cold room.leave
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