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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
AbMole
- CAS号:
55779-48-1
- 规格:
5mg
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文献和实验素和O2参与。 Gussia荧光素酶:来源于海洋桡脚类生物Gaussia princeps,是一个19.9kDa单体酶,只有185个氨基酸,具有一个16aa 的分泌信号肽,可以被细胞分泌到细胞外,反应只需要腔肠素和O2参与。 腔肠素/Coelenterazine 美国Goldbio品牌的两种腔肠素,分别是天然腔肠素/Coelenterazine及其衍生物Coelenterazine 400a。 天然腔肠素/Coelenterazine:普 遍认为是天然的腔肠荧
Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell
reaction of secreted GLase (luciferase) and coelenterazine (luciferin) are detected with an electron-multiplying charge-coupled device camera. An exocytotic event of protein secretion can be visualized using the protein fused to GLase with a time resolution
Study of GPCRProtein Interactions by BRET
resulting from the rapid oxidation of a cell-permeable coelenterazine substrate by the donor will transfer to the acceptor, which in turn fluoresces at a longer characteristic wavelength. Therefore, the occurrence of such energy transfer implies
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