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- 库存:
50
- 供应商:
AbMole
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300Tests
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文献和实验overnight. Next, slides were rinsed with water and covered with blocking solution for 2 h at room temperature. Then, after rinsing with water, AlexaFluor-488 labeled anti-mouse antibody conjugate (33 nM in blocking solution) was added to the sample slides
MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol
from DiOC1(5). The example below is a protocol for labeling with an annexin V–Alexa Fluor® 488 conjugate and propidium iodide. 1) After step 3 (above), wash cells once by adding 2 mL of warm phosphate-buffered saline or other buffer to each tube
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔: Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4412 Anti-Rabbit IgG(H+L), F(ab
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