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文献和实验range of recombinant proteins. Here, we describe the methodology associated with expressing a variety of molecular chaperones in Chinese hamster ovary (CHO) lines in order to improve their recombinant protein production capacity. These chaperones include
Generation of High-Recombinant-Protein-Producing Chinese Hamster Ovary (CHO) Cells
The purpose of this chapter is to propose a practical procedure for the generation and selection of Chinese hamster ovary cells producing high levels of recombinant protein by combining in vitro and in vivo amplification of the foreign gene
4 Make a 10X stock Night before: Make 600 ml of 1X PBS 0.1X PBS 0.1X PBS, 8% (w/w) ultrapure sucrose 8% (w/w) ultrapure sucrose LB (w/o BME): add 280 ul BME before use LB + 0.5% NP-40 (w/o BME): add 280
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