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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
AbMole
- CAS号:
1470-61-7
- 规格:
5g
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文献和实验for microbial contamination before each use if not making fresh each time. 2. Solution A: Dissolve 100 mg silver acetate (Fluka Chemical Co., catalog # 85140) in 50 ml distilled water and keep under cover. To speed dissolving the fluffy powder, stir
Zymolyase Solution: Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store aliquots at -20°C (minimize freeze-thawing if possible) Make 2.5 mg/ml zymolyase
Grow cells Harvest Spin down Wash with PBS Spin down enough cells to collect a 50-100 µL cell pellet in a FastPrep tube Prepare lysis buffer (beforehand) and chill on ice PBSMT
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