- ￥540
- Abmole
- AbMole
- M11307-100mg
- 2025年11月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
AbMole
- CAS号：
148-24-3
- 规格：
100mg
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文献和实验Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydroxyquinoline added to a final concentration of 0.1%, and equilibrated three times with an equal volume of 1M Tris.HCl, pH 7.0. The final Tris wash is replaced with TE (10
Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydroxyquinoline added
DNA Purification and Precipitation
1.Prepare or obtain Buffered phenol,pH 8.0.Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation.This also identifies the organic phase as yellow-colored. 2.Combine DNA sample with an equal volume of Buffered Phenol. 3.Mix well by vortexing
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