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- Abmole
- AbMole
- M10164-5mg
- 2025年10月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
AbMole
- CAS号：
138614-30-9
- 规格：
5mg
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文献和实验Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
and dissolve the precipitate by gentle inversion. 10. Add 4 ml of 4 M Lithium Acetate and incubate on ice for 20 min. 11. Centrifuge at 1,000 X g for 10 min. 12. Transfer the supernatant to a fresh tube, add 16 ml of 100% Ethanol, and incubate
Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
Electroelution of DNA from Agarose
in trough 2. Remove all air bubbles, then layer 80 μl of acetate cushion 3. Electroelute at: 120V for ~1Kb to 140V for >2.5Kb for 40 min for ~1Kb to 60 min for >2.5Kb 4. Collect ~300 μl of salt cushion, add 3X volumes of 95% ethanol to precipitate
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