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咨询
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- 英文名:
SIGMA
- 库存:
999
- 供应商:
麦格生物
- CAS号:
无
- 规格:
P3430-.2ML
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文献和实验Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
Monoclonal Antibody-Based Strategies in Autoimmunity and Transplantation
of therapeutic monoclonal antibodies in the fields of transplantation and autoimmunity, and discuss the salient features of antibodies presently used in the clinic-notably anti-T-cell and anticytokine antibodies. The discussion will also focus on the unique
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
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