274/85/1 [1,2,4]三唑并[1,5-a]吡.啶

In Vivo Mice The LPS-induced acute lung inflammation model Mice were randomly divided into different groups: saline group, model group (LPS), DAS treatment groups and DAS prevention groups (delivered by either intratracheal instillation or intravenous injection, respectively). The LPS group was treated with 4 μg of LPS per mouse and the saline group was given the same volume of saline. The DAS prevention groups were administered with 25 mg/kg DAS at different time interval (e.g. 1 and 6 h) before LPS challenge. Mice were sacrificed at 6 h after LPS instillation and then the BALF were collected. The DAS treatment groups were administrated with LPS and the DAS (25 mg/kg) were delivered 1 h later. Mice were sacrificed at 6 h after LPS challenge and then the BALF were collected. The BALF was collected 3 times with 0.7 mL of saline each time, and the total volume of ~2 mL was recovered. The BALF samples were centrifuged (4 °C, 1000 rpm, 15 min) and the supernatant was stored at −8139 °C for subsequent analysis. 来源文献：Wei-Ya C, Yuan-Song W, Chun-Yu L, Yu-Bin J, Fei-Fei Y, Yong-Hong