二磷酸腺苷 ADP 58-64-0

二磷酸腺苷 ADP 58-64-0 二磷酸腺苷 ADP 58-64-0
产品名称：二磷酸腺苷 ADP 58-64-0
产品编号：A1209
中文别称 二磷酸腺苷 ADP 58-64-0
英文别称 Adenosine 5'-diphosphate，ADP
CAS 58-64-0
分子式 C10H15N5O10P2
分子量 427.2
储存条件 -20°C
溶解性 50mg/ml water
纯度 ≥98％
性状 白色或类白色粉末状；无臭
包装 瓶
规格 250mg
描述 是一种含有两个磷酸基团的腺嘌呤核苷酸,具有生物或化学活性。(It is an adenine nucleotide containing two phosphate groups and is biologically or chemically active.)
靶点 Others
通路 Others
生物活性 Adenosine diphosphate is an adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.[1]
In Vitro Platelet aggregation studies. Aggregation was measured at 37°C by a turbidimetric method in a dual-channel Payton aggregometer (Payton Associates, Scarborough, Ontario, Canada). A 450-μL aliquot of platelet suspension was stirred at 1,100 rpm and activated by addition of different agonists, in the presence or abs.ence of A2P5P or A3P5P at varying concentrations and in the presence of human fibrinogen (0.8mg/mL), in a final volume of 500 μL. The extent of aggregation was estimated quantitatively by measuring the maximum curve height above baseline level. ADP-induced shape change was determined turbidimetri_x005fcally in the presence of 5 mmol/L ethylenediaminetetraacetic acid(EDTA).[2]
激酶实验 Measurement of adenylyl cyclase activity. A 450-μL aliquot of washed platelets was stirred at 1,100 rpm in an aggregometer cuvette, and the following reagents were added at 30-second intervals: 1 μmol/L PGE1, 100 μmol/L A2P5P or A3P5P, and 5 μmol/L ADP or vehicle (Tyrode’s buffer containing no Ca21 or Mg21 ). One minute later, the reaction was stopped by addition of 50 μL of ice-cold 6.6 N perchloric acid. B10 cells grown in 6-well tissue culture clusters were first incubated in BSS supplemented with 10-4 mol/L IBMX for 10 minutes at 37°C. Forskolin (1 μmol/L) and/or nucleotides were added (final volume 1 mL per well) and incubation was continued for 5 minutes at 37°C, after which the incubation solution was removed by aspiration and the cells extracted in 10% (vol/vol) ice-cold 6.6 N perchloric acid. The same procedure was applied to Jurkat cells except that the incubation solution was eliminated by centrifuging each tube at 200g for 30 seconds before extracting the cells in perchloric acid. Perchloric acid extracts were centrifuged at 11,000g for 5 minutes to eliminate protein precipitate and cyclic AMP was isolated from the supernatants as described by Khym37 using a mixture of trioctylamine and freon (28/22, vol/vol). The upper aqueous phase was lyophilized and the dry residue dissolved in the buffer provided with the commercial radioimmunoassay kit for cyclic AMP [2]

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