Orlistat (40μM; 2 days) does not affect MGMT levels in a human melanoma cell line, but downregulates the repair protein by 30-70% in human peripheral blood mononuclear cells, in two leukemia and two colon cancer cell lines. Orlistat does not alter noticeably MGMT mRNA expression. Western Blot Analysis
Cell Line:
The human melanoma cell line M10, Peripheral blood mononuclear cells , The human Jurkat CD4+T cell leukemia cell line, the human promyelocytic leukemia cell line HL60, the epithelial colon cancer HCT116 cells,non adherent mononuclear cells (NAMNC)
Concentration:
2.5, 5, 10, 20, 40μM for Jurkat cells; 20 and 40μM for HCT116 cells;40μM for normal NAMNC,M10 melanoma, HL-60 promyelocytic leukemia, and HT-29 colon cancer cells
Incubation Time:
2 days for Jurkat cells;2 or 4 days for HCT116 cells;2 days for NAMNC,M10 melanoma,HL60 promyelocytic leukemia,HT-29 colon cancer
Result:
Reduced by >50% the MGMT level at the concentration of 40μM for Jurkat cells, whereas little or no effect was found when lower concentrations were used.Downregulation of MGMT expression is produced at 40μM for HCT116 cells.Provoked an ~50% reduction of MGMT level at 40μM in normal NAMNC, and HL-60 promyelocytic leukemia, HT-29 colon cancer cells except for melanoma M10 cells that showed no downregulation of the protein.
体内研究
Orlistat (10mg/kg/day) significantly improves lipid profile, increases antioxidant enzymes and expression of antiinflammatory markers, and decreases the expression of the pro-inflammatory marker compared to the obese (OB) group.
Animal Model:
Eighteen male rats of Sprague–Dawley strain aged between 8–10 weeks weighing 200-250g
Dosage:
10mg/kg/day
Administration:
Orally;six weeks
Result:
Treatment persistently restored the increased body weight, which was significantly obs.erved at the ninth week until the end of the experimental period.
Ren L, Liu J, Liu C, Yang T, Wu X, Zhang X, Yang L, Xia J, Li W. AIn VitroCell(RAW264.7 cells;100 ng/mL LPS,24 h at 37 °C)RAW264.7 cells were seeded in confocal dishes at a density of 1 × 105 cells per dish. After the culture of 24 h, cells were treated with 100 ng/mL LPS in different media, including a control medium, PACDFe extract, and PACDFe hydrogel extract supplemented with 1 μM LL-37 for another 24 h at 2917 °C. 更多文献信息请详询客服