大家都在搜
手机验证
RT/2-8℃/-20℃
3年
Ubenimex
999
北京康瑞纳生物科技有限公司
58970-76-6
250mg
体内(现配现用): | 1.请依序添加每种溶剂:10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥0.83mg/mL(2.69mM);Clear solution |
||||
此⽅案可获得≥0.83mg/mL(2.69mM,饱和度未知)的澄清溶液。以1mL⼯作液为例,取100μL8.3mg/mL的澄清DMSO储备液加到400μL PEG300中,混合均匀;向上述体系中加⼊50μL Tween-80,混合均匀;然后继续加⼊450μL⽣理盐⽔定容⾄1mL。 | |||||
2.请依序添加每种溶剂:10% DMSO→90% (20% SBE-β-CD in saline) Solubility:≥0.83mg/mL(2.69mM);Clear solution |
|||||
此⽅案可获得≥0.83mg/mL(2.69mM,饱和度未知)的澄清溶液。以1mL⼯作液为例,取100μL8.3mg/mL的澄清DMSO储备液加到900μL20%的SBE-β-CD⽣理盐⽔⽔溶液中,混合均匀 | |||||
3.请依序添加每种溶剂:10% DMSO→90% corn oil Solubility:≥0.83mg/mL(2.69mM);Clear solution |
|||||
此⽅案可获得≥0.83mg/mL(2.69mM,饱和度未知)的澄清溶液,此⽅案不适⽤于实验周期在半个⽉以上的实验。以1mL⼯作液为例,取100μL8.3mg/mL的澄清DMSO储备液加到900μL⽟⽶油中,混合均匀。 |
Kinase Assay | Cells are harvested, washed, and lysed in NP-40 lysis buffer (50mM Tris-HCl [pH 7.5], 150mM NaCl, 0.5% NP-40). Total cell protein is quantified using the Bradford assay and 1-mg/mL protein aliquots are made. Ten microliters of total cell protein is mixed with 290 μL of substrate solution (0.1mg/mL dithiothreitol [DTT], 0.1mg/mL albumin, and 1mM alanine-β- naphthylamide). Fluorometric measurements (340nm excitation, 400nm emission) are made after 15 and 30 min. The slope of the line between the 15- and 30-min measurements is used to represent aminopeptidase activity. Total cell protein is preincubated with bestatin, amastatin, puromycin, EDTA, and/or ZnCl2 for 20 min before the fluorometric aminopeptidase assay. |
Cell Assay | Growing cells (1×106 to 2×106 cells/mL) are diluted to 1.0×103 cells/mL and transferred (3mL) into a well in a 12-well multiwell plate (2.5-cm diameter/well). Cells are treated with 0, 10, 50, 100, 300, or 600μM Bestatin and allowed to grow at 21°C shaking at 180 rpm for 48 h. A hemocytometer is used to measure cell density after 0, 24, and 48 h. |
Animal Administration | Bestatin is dissolved in PBS. The agent (doses of 10, 1, and 0.1mg/kg) is injected i.p. to non-cyclophosphamide-treated mice, 5 or 10 times at 24-h intervals before SRBC immunization. The mice are immunized 24 h after the last dose of bestatin. Pharmacological immunosuppression is induced by a single intraperitoneal injection of cyclophosphamide administered at a dose of 350mg/kg, 12 days before SRBC immunization. Bestatin at the doses of 1 and 0.1mg/kg is injected to cyclophosphamide-immunosuppressed mice i.p. five times at 48-h intervals or 10 times at 24-h intervals before SRBC immunization. The first dose of bestatin is administered 24 h after cyclophosphamide, while the last dose of the drug is injected 24h before SRBC immunization. |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
需要更多技术资料 索取更多技术资料
微信图片_20230511112833.png 附 (下载 0 次)