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RT/2-8℃/-20℃
3年
Tween 80
999
北京康瑞纳生物科技有限公司
9005-65-6
100ml
吐温80(适用于细胞培养) 9005-65-6 吐温80(适用于细胞培养) 9005-65-6
产品名称:吐温80 9005-65-6
产品编号:A3204
中文别称 吐温80;聚氧乙烯山梨糖醇酐单油酸酯;吐温-80;Polyethylene glycol sorbitan monooleate; Polyoxyethylenesorbitan monooleate;Polysorbate 80。
英文别称 Tween-80
CAS 9005-65-6
储存条件 RT
有效期 1年
描述 Tween 80是一种非离子型表面活性剂。(Tween 80 is a nonionic surfactant.)
生物活性 Tween 80 是一种表面活性剂,可以减少细菌附着并抑制生物膜的形成。[1-2]
In Vitro 吐温80是一种表面活性剂,它还可以减少细菌附着并抑制生物膜形成[1]。在体外使用1%吐温80可以实现高达100倍的溶解度增强[2]。
性状 无色至淡黄色粘稠液体
包装 瓶
规格 100ml 500ml
说明:为非离子型亲水性表面活性剂。气相色谱固定柱(最高使用温度160℃,溶剂为氯仿、二氯.甲烷、甲醇),分离分析脂肪酸酯、醇、酮和卤化物;扩散剂;湿润剂。
吐温(或聚山梨酯)为非离子型表面活性剂,是山梨醇与不同高级脂肪酸所形成的酯。有异臭、微苦。一般呈淡黄色到琥珀色粘稠液体状,但因分子量差异而有所不同。酸碱度:约6-8(5%)
根据不同高级脂肪酸所形成的酯,吐温分20,40,60,80等多种,根据不同的需要来选用。常用的有吐温20(Tween-20)、吐温21((Tween -21)、吐温40((Tween -40)、吐温60((Tween -60)、吐温61(T(Tween -61)、吐温80(T(Tween 80)、吐温81(Tween -81)、吐温85((Tween -85) 。
吐温60是硬脂酸酯;吐温 80为油酸酯;吐温20为月桂酸酯,是聚氧乙烯去水山梨醇单月桂酸酯和一部分聚氧乙烯双去水山梨醇单月桂酸酯的混合物
主要应用为:
1. Tween 和同类型的Triton 100(聚乙二醇辛基苯基醚)非离子型去污剂可以在生物学实验中乳化蛋白,不破坏蛋白的结构,可减少对蛋白质之间原有的相互作用的破坏。离子型去污剂如SDS则破坏蛋白的结构。
2. 在生物学实验中作封闭剂,封闭所有未结合位点而不替换膜上的靶蛋白、不结合靶蛋白的标位、也不与抗体或检测试剂有交叉反应。Tween-20有复性抗原的作用,可提高特异性的识别能力。在做western blot时,用惰性蛋白质或非离子去污剂封闭膜上的未结合位点,可以降低抗体的非特异性结合。最常见的封闭剂是BSA、脱脂奶粉、酪蛋白、明胶和Tween-20(0.05 – 0.1%)稀溶液。
3.常作为水包油(O/W)型乳化剂,使其他物质均匀在溶液中分散,主要用于农药、食品、化妆品。相对来说,吐温20更温和一些,吐温80的乳化性更强一些。
4.采用过滤法进行单核细胞增多性李斯特氏菌计数
5.作为制备W1/O/W2乳状液的亲水性乳化剂
6.作为牛结核分.枝杆菌卡介苗(BCG)巴斯德菌株培养中7H9 +油酸清蛋白葡萄糖过氧化氢酶的组成部分
7.非离子型洗涤剂,用于哺乳动物细胞系中选择性蛋白质提取和细胞核分离。
Tween 80 (for cell culture) 9005-65-6 Tween 80 (for cell culture) 9005-65-6
Product Name: Tween 80 9005-65-6
Product number: A3204
Also known as Tween 80 in Chinese; Polyoxyethylene sorbitol anhydride monooleate; Tween-80; Polyphylene glycol sorbitan monooleate; Polyoxyethylenesorbitan monooleate; Polysorbate 80.
English alias Tween-80
CAS 9005-65-6
Storage conditions RT
Validity period of 1 year
Tween 80 is a non-ionic surfactant. (Tween 80 is a nonionic surfactant.)
Bioactive Tween 80 is a surfactant that can reduce bacterial attachment and inhibit biofilm formation. [1-2]
In Vitro Tween 80 is a surfactant that can also reduce bacterial attachment and inhibit biofilm formation [1]. The use of 1% Tween 80 in vitro can achieve up to 100 times enhanced solubility [2].
Colorless to light yellow viscous liquid in appearance
Packaging bottle
Specification 100ml 500ml
Explanation: It is a non-ionic hydrophilic surfactant. Gas chromatography fixed column (with a maximum operating temperature of 160 ℃ and solvents of chloroform, dichloromethane, methanol) for separation and analysis of fatty acid esters, alcohols, ketones, and halides; Diffuser; Moisturizing agent.
Tween (or polysorbate) is a non-ionic surfactant, which is an ester formed by sorbitol and different higher fatty acids. Has a foul odor and slight bitterness. It generally appears as a light yellow to amber viscous liquid, but varies due to differences in molecular weight. PH: approximately 6-8 (5%)
According to the esters formed by different advanced fatty acids, Tween can be divided into various types such as 20, 40, 60, 80, etc., and selected according to different needs. Commonly used ones include Tween-20, Tween-21, Tween-40, Tween-60, Tween-61, Tween-80, Tween-81, Tween-81, and Tween-85.
Tween 60 is a stearic acid ester; Tween 80 is an oleate ester; Tween 20 is a laurate, a mixture of polyoxyethylene dehydrated sorbitol monolaurate and a portion of polyoxyethylene di dehydrated sorbitol monolaurate
The main applications are:
1. Tween and the same type of Triton 100 (polyethylene glycol octylphenyl ether) nonionic detergent can emulsify proteins in biological experiments without damaging their structure and reducing the damage to the original interactions between proteins. Ionic detergents such as SDS destroy the structure of proteins.
2. In biological experiments, act as a blocking agent to block all unbound sites without replacing the target protein on the membrane, unbound targets, and without cross reactivity with antibodies or detection reagents. Tween-20 has the function of refolding antigens, which can improve specific recognition ability. When performing Western blot, blocking the unbound sites on the membrane with inert proteins or non-ionic detergents can reduce the non-specific binding of antibodies. The most common sealants are BSA, skim milk powder, casein, gelatin, and Tween-20 (0.05-0.1%) dilute solution.
3. Often used as an oil in water (O/W) emulsifier to evenly disperse other substances in solution, mainly used in pesticides, food, and cosmetics. Relatively speaking, Tween 20 is milder, while Tween 80 has stronger emulsifying properties.
4. Use filtration method for counting Listeria monocytogenes
5. As a hydrophilic emulsifier for preparing W1/O/W2 emulsions
6. As a component of 7H9+oleic acid albumin glucose catalase in Pasteur strain culture of Mycobacte.rium tuberculosis BCG vaccine
7. Non ionic detergent, used for selective protein extraction and cell nucleus separation in mammalian cell lines.
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Ren L, Liu J, Liu C, Yang T, Wu X, Zhang X, Yang L, Xia J, Li W. AIn Vitro Cell(RAW264.7 cells;100 ng/mL LPS,24 h at 37 °C) RAW264.7 cells were seeded in confocal dishes at a density of 1 × 105 cells per dish. After the culture of 24 h, cells were treated with 100 ng/mL LPS in different media, including a control medium, PACDFe extract, and PACDFe hydrogel extract supplemented with 1 μM LL-37 for another 24 h at 1498 °C. 更多文献信息请详询客服
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