TRIS-HCl 三羟甲基（氨基）甲烷盐酸盐（适用于细胞培

TRIS-HCl  三羟甲基（氨基）甲烷盐酸盐（适用于细胞培养） 1185-53-1 TRIS-HCl  三羟甲基（氨基）甲烷盐酸盐（适用于细胞培养） 1185-53-1
产品名称：TRIS-HCl  三羟甲基（氨基）甲烷盐酸盐 1185-53-1
产品编号：A3172 中文别称 盐酸三(羟甲基)氨基.甲烷； 2-Amino-2-(hydroxymet.hyl)-1,3-propanediol hydrochloride；TRIS hydrochloride； Tromethane hydrochloride。
英文别称 Tris (Hydroxyme.thyl) Aminomethane Hydrochloride
CAS 1185-53-1
分子式 C4H12ClNO3
分子量 157.64
储存条件 RT
有效期 5年
级别 Ultra Pure Grade
溶解性 667mg/ml in water
危险代码 36/37/38
纯度 Purity≥99.9%。
性状 白色结晶粉末
包装 瓶
规格 100g 500g
质量标准：

外观：白色结晶
含量：≥99.0％
熔点：150.0-153.0℃
Residue on Ignition：≤0.1％
水溶性：清澈
水分：≤0.5％
PH(1.0mol/L)：3.7-4.7
PKa(20℃)：8.0-8.4
说明
Tris-HCl，也称为Trizma®盐酸盐，是一种广泛使用的酸性缓冲液，用于制备生理pH范围为7.3至7.5的缓冲液。Tris-HCl在25°C下的pKa为8.08，其pH范围为7.0至9.0，与生物体的典型pH相匹配。因此，Tris-HCl是用于各种生物、细胞培养、生物化学和分子生物学应用的缓冲溶液的重要成分。需要注意的是，无论是Tris碱还是Tris盐酸盐本身都不能提供足够的缓冲能力。因此，它们通常被组合以产生pH范围为7-9的缓冲液，从而确保有效的pH控制。
Tris缓冲液在蛋白质电泳和蛋白质印迹等技术中是必不可少的。在许多蛋白质印迹方案中，低离子强度的Tris缓冲液用于蛋白质转移。转移持续时间取决于印迹设备和所研究的肽的大小范围。基于Tris的缓冲液也常用于SDS-PAGE凝胶、运行缓冲液和印迹缓冲液中。
此外，Tris盐在DNA琼脂糖电泳中得到了广泛的应用，特别是在制备缓冲液如TAE（Tris-acetate/EDTA）和TBE（Tris-硼酸盐/EEDTA）中。这些缓冲液在各种应用中发挥着关键作用，包括生物化学、蛋白质纯化和电泳。Tris-HCl是一种多用途且必不可少的物质，用于维持生物系统的pH值，并确保生物化学、细胞培养和分子生物学技术的最佳性能。

应用
已使用Tris-HCl
在免疫组织化学分析中作为洗涤和漂洗缓冲液（1）
作为还原样品缓冲液、裂解缓冲液和洗脱缓冲液的组成部分，在涉及凝胶电泳和BioLayer干涉测量的生化研究中（2）
特点和优势：
适用于分子生物学和细胞培养
可作为缓冲组分，用于电泳和蛋白质分离
内毒素和总需氧微生物计数测试
不含DNase、NICKase、RNase和蛋白酶
经过测试，确认重金属污染水平较低，确保适用于各种应用从pH 7-9（25°C）到pKa为8.1（25℃）的有效缓冲高度溶于水

产品应用：Tris-HCL缓冲液不仅被广泛用作核酸和蛋白质的溶剂，还有许多重要用途。Tris-HCL被用于不同pH条件下的蛋白质晶体生长。[3]Tris-HCL缓冲液的低离子强度特点可用于线虫（C. elegans）核纤层蛋白（lamin）的中间纤维的形成。[4]Tris-HCL也是蛋白质电泳缓冲液的主要成分之一。此外，Tris-HCL还是制备表面活性剂、硫化促进剂和一些药物的中间物。Tris-HCL也被用作滴定标准物。
explain
Tris HCl, also known as Trizma ® Hydrochloride is a widely used acidic buffer used to prepare buffer solutions with a physiological pH range of 7.3 to 7.5. The pKa of Tris HCl at 25 ° C is 8.08, with a pH range of 7.0 to 9.0, which matches the typical pH of the organism. Therefore, Tris HCl is an important component of buffer solutions used in various biological, cell culture, biochemical, and molecular biology applications. It should be noted that neither Tris base nor Tris hydrochloride itself can provide sufficient buffering capacity. Therefore, they are typically combined to produce a buffer solution with a pH range of 7-9, ensuring effective pH control.

Tris buffer is essential in techniques such as protein electrophoresis and Western blotting. In many protein imprinting schemes, low ion strength Tris buffer is used for protein transfer. The duration of transfer depends on the imprinting equipment and the size range of the studied peptide. Tris based buffer is also commonly used in SDS-PAGE gel, running buffer and imprinting buffer.

In addition, Tris salts have been widely used in DNA agarose electrophoresis, especially in the preparation of buffer solutions such as TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA). These buffers play a crucial role in various applications, including biochemistry, protein purification, and electrophoresis. Tris HCl is a versatile and essential substance used to maintain the pH value of biological systems and ensure optimal performance in biochemical, cell culture, and molecular biology technologies.
application

Tris HCl used

As washing and rinsing buffer in immunohistochemical analysis (1)

As a component of reduced sample buffer, lysis buffer and elution buffer, in biochemical studies involving gel electrophoresis and BioLayer interferometry (2)

Features and advantages:

Suitable for molecular biology and cell culture

Can be used as a buffer component for electrophoresis and protein separation

Endotoxin and total aerobic microbial count test

Excluding DNase, NICKase, RNase, and protease

After testing, it has been confirmed that the level of heavy metal pollution is low, ensuring effective buffering and high solubility in water for various applications, ranging from pH 7-9 (25 ° C) to pKa 8.1 (25 ° C)



Product application: Tris HCL buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris HCL was used for protein crystal growth under different pH conditions. [3] The low ion strength characteristic of Tris HCL buffer can be used for the formation of intermediate fibers in the lamin of C. elegans. [4] Tris HCL is also one of the main components of protein electrophoresis buffer. In addition, Tris HCL is also an intermediate in the preparation of surfactants, vulcanization promoters, and some drugs. Tris HCL is also used as a titration standard.

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