脱氧核糖核酸酶Ⅰ 9003-98-9

脱氧核糖核酸酶Ⅰ 9003-98-9 脱氧核糖核酸酶Ⅰ 9003-98-9
产品名称：脱氧核糖核酸酶Ⅰ 9003-98-9
产品编号：A1859
中文别称 DNase I 脱氧核糖核酸 5′-寡核苷酸-水解酶
英文别称 DNase I(DeoxyribonueleaseⅠ)
CAS 9003-98-9
分子量 约31 kDa
储存条件 -20℃
有效期 3年
溶解性 0.15 M NaCl (5mg/ml)
酶活/效价 1500 units/mg
性状（性状） 白色冻干粉
包装 支
规格 25mg 100mg 1g
DeoxyribonueleaseⅠ
CAS Number : 9003-98-9
Synonyms: DNase I;Deoxyribonucleate 5’-Oligonucleotidohydrolase
Storage Temperature: -20 °C
Product Description：
DNase I is found in most cells and tissues. In mammals the pancreas is one of the best sources for the enzyme. Pancreatic DNase I was the first DNase isolated. DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5’-phosphates. A tetranucleotide is the smallest average digestion product. In the presence of Mg 2+ ions, DNase I attacks each strand of DNA independently and the cleavage sites are random. If Mn 2+ ions are present, both DNA strands are cleaved at approximately the samesite. DNase I hydrolyzes single and double-stranded DNA and chromatin (reaction rate is restricted by DNA association with histones).
DNase I is used to remove DNA from protein and nucleic acid samples, and to nickDNAas a first step to incorporate labeled bases into
DNA.
Molecular mass:30,072 Da (peptide, calculated), exists as a mixture of glycoproteins with two disulfide bridges.
Activators:
DNaseI has an absolute requirement for divalent metal cations. The most commonly used is Mg 2+ ;however, Mn 2+ , Ca 2+ , Co 2+ , and Zn 2+will activate DNaseI. A concentration of 5 mM Ca 2+will stabilize DNase I against proteolytic digestion; 0.1 mM is needed to reduce the rate of inactivation by one-half.
Inhibitors:
2-Mercaptoethanol (the reduced enzyme is inactive, but can be reactivated in the presence of Ca 2+or Mg 2+ ions); chelators; sodium dodecyl sulfate (SDS);and actin.There is no general inhibitor specific for DNase I.Citrate inhibits Mg 2+ -activated DNase I, but not Mn 2+ -activated DNase I. Preparation Instructions This enzyme is soluble in 0.15 M NaCl (5 mg/ml), yielding a clear solution.
Storage/Stability
DNase I retains activity for at least three years when stored at –20 °C. Solutions of DNase I (10 mg/ml) in 0.15 M NaCl may lose <10% of its activity stored for a week in aliquots at –20 °C. The same solutions stored in aliquots at 2–8 °C can lose ~20% activity. DNase I remains active in solution between pH 5 and 7 up to 60 °C for at least five hours. A 1 mg/ml solution in acetate buffer (pH 5.0) or Tris buffer (pH 7.2) loses activity at the rate of 6%/hour. At 68 °C DNaseI loses activity in <10 minutes.
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other uses.

溶解性:DMSO，鉴定方法:液相，熔点:102℃-260℃，沸点：522.2°C at 760 mmHg，密度:1.064 g/cm³，保存条件:-20℃/2-8℃/RT避光，运输条件:2-8℃，保质期:2年，产地:国产/进口，品牌:北京康瑞纳生物，类别:生化试剂，库存:999，用途:用于科研实验，细胞培养，体外诊断，配方原料，医药生产等方面；使用方法: 详询客服

 








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