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- BD
- 354277-5mL
- 美国
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
LDEV-free
- CAS号：
无
- 保质期：
无
- 供应商：
麦格生物
- 保存条件：
-20°C
- 规格：
5ml
品质保证,现货供应,可全国发货,质量保证,售后无忧
| 产品品牌： | BD |
| 产品名称： | Matrigel 胚胎干细胞基质胶, hESC-Qualified Matrix, LDEV-free |
| 产品货号： | 354277 |
| 产品规格： | 5mL |
| 保存温度： | -20°C |
BD Matrigel 胚胎干细胞基质胶, hESC-Qualified Matrix, LDEV-free说明：
Corning Matrigel基质是一种从Engelbreth-Holm Swarm（EHS）小鼠肉瘤中提取的可溶性基底膜制剂，该肿瘤富含细胞外基质蛋白，包括层粘连蛋白（一种主要成分）、IV型胶原、硫酸乙酰肝素蛋白多糖、entactin/nidogen和多种生长因子。
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
-Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
-Extensive PCR testing is performed to screen for a number of pathogens
-Tested and found negative for bacteria, fungi, and mycoplasma
-Protein concentrations determined by Lowry method
-Endotoxin units measured by Limulus Amoebocyte Lysate assay
-Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
-Biological activity is determined for each lot using a neurite outgrowth assay.
Source
Engelbreth-Holm-Swarm (EHS) mouse tumor
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws
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文献和实验Sodium Carbonate: A Versatile Material (2000)
Preparation of biogenic gas vesicle nanostructures for use as contrast agents for ultrasound and MRI
Lakshmanan A, et al.
Nature Protocols, 12(10), 2050-2050 (2017)
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs
, laminin, and proteoglycans)but also matrix degrading enzymes/their inhibitors and growth factors. Invasion of tumor cells into Matrigel has been used to characterize involvement of ECM receptors and matrix degrading enzymes which play roles in tumor
In Vitro Matrigel Fibroblast Invasion Assay
with a thin layer of matrigel. On top of this matrigel fibroblast-like synoviocytes are seeded at a density of 100,000 cells per milliliter. The cells are cultured in serum free medium in the inner compartment inside the transwell. To the outer compartment
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