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麦格生物
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| 产品品牌: | Millipore |
| 产品名称: | 硝酸纤维素膜33cm*3m 0.45µm |
| 产品货号: | Merck-HATF00010 |
| 产品规格: | 卷 |
| 保存温度: | 常温 |
Millipore 硝酸纤维素膜33cm*3m 0.45µm说明:
The Immobilion-NC (HATF) membrane is composed of a mixed cellulose ester matrix. It lacks surfactants and may interfere with the integrity of cell walls during cell growth. This membrane is recommended for colony and plaque enhancement.


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文献和实验10x AA-dUTP, 2 mM (MW = 523 g/mol), (Sigma A0410, $193.50/1mg - dissolve in 9.5 mL H2 O) 1 N NaOH, 0.5 M EDTA, 2 M HEPES 0.2 M NaHCO3 (bicarbonate), 4 M NH2 OH GFX spin columns QiaQuick columns PolyA (10 mg/mL) - Sterile filtered (0.45 µm
Chromatin Immunoprecipitation (ChIP)
(Millipore) Proteinase K (20 µg/uL) TE buffer (optional; see Step 30) Equipment Cell scraper Centrifuge (benchtop) Dishes or flasks (tissue culture) Gel electrophoresis apparatus Gel-loading tips (narrow
How do you synthesize your dsRNA
your selected primer sequences to amplify the region of interest. Use 1-2 µl of a 10µM primer stock, 100-200 ng DNA as template and DNA polymerase. We have successfully used GeneChoice Taq (PGC Scientifics #62-6086-01), TaqPlus (Stratagene #600210
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