WB result of Goat anti-Mouse IgG(H+L) Primary antibody: Hsp90 Mouse mAb at 1/1000 dilution Lane 1: Jurkat whole cell lysate 20 ug Secondary antibody: Goat anti-Mouse IgG(H+L), HRP conjugated at 1/10000 dilution Predicted MW: 90 kDa Observed MW: 90 kDa Exposure time: 180 s
WB result of Goat anti-Mouse IgG(H+L) Primary antibody: Hsp90 Mouse mAb at 1/1000 dilution Lane 1: rat brain lysate 20 ug Secondary antibody: Goat anti-Mouse IgG(H+L), HRP conjugated at 1/10000 dilution Predicted MW: 90 kDa Observed MW: 90 kDa Exposure time: 180 s
S0B4006 or secondary antibody from control product was used at 1/10000 dilution in ELISA Recommend Dilution: 1:5000-1:30000