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- 2025年07月13日
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文献和实验Expression of Chemokines in Escherichia coli
. Inclusion of a lac operator in the T7 promoter and the presence of a vector encoded lactose repressor, lacI, helps to reduce basal transcription levels. Background expression can be further reduced by cotransformation with plasmids encoding T7 lysozyme
Expression of Recombinant Proteins From lac Promoters
The Gram-negative bacterium Escherichia coli enjoys widespread use in modern biology as both a model organism and a microbiological tool. One of the keys to its popularity lies in the functionality of the lac operon. This regulated E. coli
Strategies to Optimize Protein Expression in E. coli
. 5:478‐487. Baneyx, F. and Mujacic, M. 2004. Recombinant protein folding and misfolding in Escherichia coli. Nat. Biotechnol. 22:1399‐1408
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