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- 2025年07月10日
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文献和实验Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase
In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification
Isolation of Recombinant DNA Elongation Proteins
This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) δ, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d
Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
sequence, the cloned DNA fragment being evaluated is inserted upstream of a particular reporter gene-containing vector. The recombinant promoter-reporter gene construct is then introduced into an appropriate cell type, either by CaPO4 transfection
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