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文献和实验Recombinant Collagen Trimers from Insect Cells and Yeast
have been unsuccessful, but active recombinant human prolyl 4-hydroxylase has been produced in insect cells and yeast by coexpression of its α- and β-subunits (7 ,7 ). We have recently shown that recombinant human type III collagen with a stable triple helix
Human Chromosome Analysis and Sorting
of small but useful quantities of individual chromosomes, for example, 2�106 average sized human chromosomes are equivalent to 500 ng of DNA. Using recombinant DNA techniques, this material can be used to generate a large number of DNA probes to produce
a procedure for the isolation of nondenatured, recombinant human AR with more than 95% purity using four-step chromatography with milligram yields (see Notes 1 -Notes 4 ). Purified AR may be used in physical and biochemical studies, such as monoclonal antibody
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