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- 2025年07月14日
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文献和实验RANKL-Based Osteoclastogenic Assays from Murine Bone Marrow Cells
or indirectly. This protocol describes a procedure for generating osteoclasts from mouse bone marrow cells using macrophage colony stimulating factor and receptor activator of NF-κB ligand.
Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
Biotinylated nuclear receptor ligand binding domain (NR LBD; non‐biotinylated receptors are commercially available from Invitrogen, Active Motif, Jena Bioscience, Protein One, as well as other manufacturers)
. Hormone treatment activates the steroid receptor, which recruits chromatin remodeling complexes (CRCs), and coactivators (CoAs), and ultimately results in a remodeling of nucleosome B to an active chromatin architecture that permits transcription factor
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