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文献和实验unsuccessful, emphasizing a need for further refinement. A novel method, the complete open reading frame (C-ORF) technique, is presently described, which has proven successful in cases, where standard rapid amplification of cDNA ends (RACE) has not worked. In C
RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus
in this chapter. (A ) HCV genome comprising 5′ and 3′ untranslated regions (bold lines), flanking an open reading frame (box). Nucleotide (upper, italics), and amino acid (lower, plain) scales are shown. (B ) Enlarged envelope 2 (E2) and nonstructural 5A (NS
Typical transformation results
Transformation results for ORF YER141W Gene name = COX15 Gene product = cytochrome oxidase assembly factor Previous disruption = unknown This open reading frame might be a good candidate for an essential gene, based on transformation results. Recent
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