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文献和实验Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport
Shiga toxin, produced by Shigella dysenteriae and Shiga toxin-producing Escherichia coli , is a bacterial protein toxin composed of two subunits, A and B (1 ). The A-subunit is the actual toxin whose ribosomal RNA N -glycosidase activity
Isolation of Recombinant DNA Elongation Proteins
This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) δ, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d
Strategies to Optimize Protein Expression in E. coli
transporter CorA overexpressed in Escherichia coli. Protein Expr. Purif. 32:221‐231. Choi, S.I., Song, H.W., Moon, J.W., and Seong, B.L. 2001. Recombinant enterokinase light chain
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