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- Biomatik
- RPU43208
- 2025年07月06日
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文献和实验Phage-Displayed Single Domain Antibodies as Recognition Elements
The variable domains of antibodies can be expressed as a fusion with pIII, a minor coat protein of the bateriophage M13, for the generation of phage-display antibody reagents. The phage-display system is routinely used to enrich
An Improved Method for Mapping Epitopes of Recombinant Antigens by Transposon Mutagenesis
are determined by transposon-primed DNA sequencing The predicted sizes of the N-terminal fragments of the target protein are ranked in decreasing size. The N-terminal fragments are expressed in E. coli and tested for recognition by a panel of MAbs. Epitopes
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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