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文献和实验Assay for Human Rad51-Mediated DNA Displacement Loop Formation
as described by Chi et al. (2007) . Purify Rad54 as described by Sigurdsson et al. (2002) . Recombinant human Rad51, purified (5-10 µM) Purify as described by Sigurdsson et al. (2001) . SDS (Sodium dodecyl sulfate;
Biochemical Studies on Human Rad51-Mediated Homologous Recombination
Rad51-mediated pairing between homologous DNA sequences during homologous recombination (HR) plays pivotal roles in DNA double-strand break repair. The multi-step process occurs through cooperation of Rad51 and a number of accessory protein
In Vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis
/Rad51 family of proteins are the key enzymes in this homology-directed repair pathway, as they perform DNA strand invasion and exchange, in concert with a host of ancillary factors. In vitro, the RecA/Rad51 family of proteins share similar enzymatic
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